Product Description
50 Units
Zymo E2018-50″ title=”dsDNA Shearase Plus, 1 U/ul,
50 Units
Zymo E2018-50″ />dsDNA Shearase Plus, 1 U/ul,
50 Units
Zymo E2018-50
dsDNA Shearase Plus is an endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5′-phosphates and 3′-hydroxyl termini. It has a particularly strong preference for dsDNA and generates random-ended DNA fragments of the desired size in a single step. This enzyme is compatible with low volume inputs thus minimizing sample loss.
The simplest method for generating random-ended dsDNA fragments.Fragment size is conveniently controlled by adjusting the enzyme concentration.dsDNA Shearase Plus-generated fragments are ideal for library construction, Next-Gen sequencing, and methylated DNA immunoprecipitation (MeDIP).
Unit Definition: One unit (U) is defined as the amount of enzyme required to convert 250 ng human DNA into DNA fragments in the range of 100 – 500 bp in 20 minutes at 42°C in a total reaction volume of 10 µl.Specificity: Provided with 5X dsDNA Shearase Plus Reaction BufferEnzyme Inactivation and DNA Cleanup: For enzyme inactivation and DNA purification we recommend the DNA Clean & Concentrator kit. Addition of 5 volumes of DNA Binding Buffer from the DNA Clean & Concentrator kit to each reaction volume (5:1) inactivates dsDNA Shearase Plus. Alternatively, the enzyme can be inactivated by incubating at 65°C for 5 minutes followed by DNA purification by phenol/chloroform extraction.Enzyme Concentration: 1 U/µlEnzyme Inactivation: 65°C for 5 min.Optimum Reaction Temperature: 42°C
