Product Description
1000 Units
Zymo E1020-1000″ title=”PureRec Duplex-Specific Nuclease (DSN)
1000 Units
Zymo E1020-1000″ />PureRec Duplex-Specific Nuclease (DSN)
1000 Units
Zymo E1020-1000
HIGHLIGHTS :
Highest Specificity: Selectively digests dsDNA and DNA in DNA-RNA hybrids; virtually inactive towards ssDNA or RNA.Premium Purity: RNase-free, protease-free, and animal-free.Thermostable: Able to withstand high temperatures without denaturation or loss in activity.
DESCRIPTION :
PureRec Duplex-Specific Nuclease (DSN) is an endonuclease that selectively digests double-stranded DNA (dsDNA) and DNA in DNA-RNA hybrids, exhibiting virtually no activity towards single-stranded DNA (ssDNA) or RNA.
It can discriminate between perfectly matched and non-matched short DNA duplexes. To catalyze cleavage, DSN needs a minimum of 10 bp dsDNA or 15 bp perfectly matched DNA-RNA duplex.
This enzymatic function is reliant upon the presence of Mg2+ ions, with a recommended concentration of 5 mM for most applications.
PureRec DSN is overexpressed and extensively purified from a non-animal source and is free of non-specific nucleases or RNases.
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APPLICATION :
Remove gDNA and DNA in DNA:RNA hybrids for protein or RNA preparations.Reduce abundant rRNA and globin transcripts to enhance next-generation sequencing (NGS) libraries.Achieve precise single-nucleotide polymorphism (SNP) detection with minimal off-target effects.Enhance signal amplification for accurate virus detection.Improve detection of miRNA and biomarkers.
