Product Description
96 preps
Zymo R2137″ title=”ZymoBIOMICS MagBead RNA,
96 preps
Zymo R2137″ />ZymoBIOMICS MagBead RNA,
96 preps
Zymo R2137
The ZymoBIOMICS MagBead RNA kit provides a high-throughput, magnetic beadbased purification of total RNA (including small/microRNAs) from any sample source (e.g., microbes including gram positive/negative bacteria, fungi, protozoans, viruses, fecal, soil, plant, water, biofilms, swabs, saliva, body fluids and etc.). The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. Total RNA is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water and is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Unbiased Lysis: Efficient and unbiased lysis of microbes including gram positive/negative bacteria, fungi, protozoans, and viruses from any sample including feces, soil, plant, water, biofilms, swabs, saliva, body fluids, etc.Ultra-Pure: High-quality total RNA (including small/microRNAs) is inhibitor-free and ready for RT-PCR and microbiome measurements using Next-Gen Sequencing.High-Sensitivity: Increased detection limit of very low abundance organisms.
Binding Capacity: 15 µg total RNA per 30 µl MagBinding Beads.Equipment Needed: Magnetic stand, heat block, liquid handler or robotic sample processor (user provided).Purity: High-quality RNA is ready for Next-Gen Sequencing, RT/PCR, hybridization, etc.Recommended Materials: (sold separately) – 96-well Collection Plate (C2002; capacity is up to 1.2 ml/well), 96-Well Block (P1001; capacity is up to 2 ml/well), 96-well Elution Plate (C2003), Cover Foil (C2007), ZR-96 MagStand (P1005).Sample Preservation: DNA/RNA Shield lyses cells, inactivates nucleases and infectious agents and is ideal for safe sample storage and transport at ambient temperatures.Sample Types: Bacterial, fungal, protozoan, algae, viral, mitochondrial, and host DNA and RNA are efficiently isolated from ≤ 50mg of mammalian feces, ≤ 50mg soil, and 5-20mg (wet weight) of fungal bacterial cells, biofilms, water, and swabs.Size Limits: Total RNA including small/microRNAs ≥ 17nt.Storage: RNA eluted with DNase/RNase-Free Water (provided) can be stored frozen. The addition of RNase inhibitors is highly recommended for prolonged storage.
